2PM9

Crystal structure of yeast Sec13/31 vertex element of the COPII vesicular coat

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	200
Detector technology	CCD
Collection date	2006-06-20
Detector	ADSC QUANTUM 4
Spacegroup name	P 43
Unit cell lengths	155.163, 155.163, 59.866
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 3.300
Rwork	0.251
R-free	0.30500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	yeast Sec13
RMSD bond length	0.009
RMSD bond angle	1.636
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.420
High resolution limit [Å]	3.300	3.300
Rmerge	0.092	0.418
Number of reflections	20444
<I/σ(I)>	11.9	2.1
Completeness [%]	92.6	76.3
Redundancy	2.7	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	295	8% PEG 3350, 0.1M tri-sodium citrate, 10mM manganese chloride, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K