2PLZ
Arg-modified human beta-defensin 1 (HBD1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.96 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 20.366, 27.408, 55.079 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.360 |
R-factor | 0.10776 |
Rwork | 0.105 |
R-free | 0.15797 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2IJV |
RMSD bond length | 0.020 |
RMSD bond angle | 1.963 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.410 |
High resolution limit [Å] | 1.360 | 1.360 |
Rmerge | 0.087 | 0.341 |
Number of reflections | 7002 | |
<I/σ(I)> | 17 | 4.2 |
Completeness [%] | 99.6 | 99.4 |
Redundancy | 5.7 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.0 M Lithium sulfate, 2% (w/v) PEG8000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |