2PK9

Structure of the Pho85-Pho80 CDK-cyclin Complex of the Phosphate-responsive Signal Transduction Pathway

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	200
Detector technology	CCD
Collection date	2005-07-21
Detector	CUSTOM-MADE
Wavelength(s)	0.97948
Spacegroup name	P 31 2 1
Unit cell lengths	147.775, 147.775, 211.825
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.030 - 2.906
R-factor	0.28316
Rwork	0.281
R-free	0.31536
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.469
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.030	3.000
High resolution limit [Å]	2.906	2.906
Rmerge	0.096	0.290
Number of reflections	47431
<I/σ(I)>	12.2	2.4
Completeness [%]	88.4	71.2
Redundancy	3.2	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	283	9ul 12% PEG 10000, 10% glycerol, 0.2ul 0.25 M strontium chloride (SrCl2), 10 mM Tris (2-carboxyethyl) phosphine (TCEP, 0.1 M 2-morpholinoethanesulfonic acid (MES), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K
1	VAPOR DIFFUSION, HANGING DROP	6.5	283	9ul 12% PEG 10000, 10% glycerol, 0.2ul 0.25 M strontium chloride (SrCl2), 10 mM Tris (2-carboxyethyl) phosphine (TCEP, 0.1 M 2-morpholinoethanesulfonic acid (MES), pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K