2PEH
Crystal structure of the UHM domain of human SPF45 in complex with SF3b155-ULM5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.920, 66.380, 72.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.840 - 2.110 |
| R-factor | 0.21132 |
| Rwork | 0.208 |
| R-free | 0.27272 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB-ID 2pe8 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.385 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.300 | 2.170 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.049 | 0.206 |
| Number of reflections | 13008 | |
| <I/σ(I)> | 32.56 | 9.46 |
| Completeness [%] | 89.3 | 78.5 |
| Redundancy | 7.1 | 6.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 25% (w/v) PEG 1,500, 0.1 M Malonate, Imidazole, Borate buffer pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
