2PBO

E27Q mutant of EXO-B-(1,3)-Glucanase from Candida Albicans at 1.85 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1998-11-02
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	60.000, 65.170, 96.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.440 - 1.850
R-factor	0.131
Rwork	0.130
R-free	0.15800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1eqp
RMSD bond length	0.011
RMSD bond angle	1.231
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.040	0.025	0.144
Number of reflections	32687
<I/σ(I)>	13.4
Completeness [%]	91.1	81.7	91.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	293	PEG 8000, Hepes, CaCl2, pH 7.3, temperature 293K, VAPOR DIFFUSION, HANGING DROP