2P6X
Crystal structure of human tyrosine phosphatase PTPN22
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97900 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.961, 48.442, 119.805 |
Unit cell angles | 90.00, 102.84, 90.00 |
Refinement procedure
Resolution | 59.440 - 1.900 |
R-factor | 0.16627 |
Rwork | 0.164 |
R-free | 0.21725 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2oc3 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.518 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.440 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.080 | 0.389 |
Number of reflections | 54178 | |
<I/σ(I)> | 13.1 | 3.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 293 | 100 mM Bicine pH 7.9, 25% PEG 10000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |