2P4E
Crystal Structure of PCSK9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-01 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.810, 70.670, 150.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 75.000 - 1.980 |
R-factor | 0.202 |
Rwork | 0.200 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ic6 |
RMSD bond angle | 0.011 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.000 | 2.050 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.115 | 0.570 |
Number of reflections | 46285 | |
<I/σ(I)> | 15.4 | 2 |
Completeness [%] | 97.9 | 84.4 |
Redundancy | 6.6 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 300 | 25% PEG 1500, 50 mM Tris-HCl buffer, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |