2P49
Complex of a camelid single-domain vhh antibody fragment with RNASE A at 1.4A resolution: native mono_1 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.130, 54.394, 48.044 |
| Unit cell angles | 90.00, 108.49, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.380 |
| R-factor | 0.15714 |
| Rwork | 0.156 |
| R-free | 0.18452 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bzq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.656 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.430 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Number of reflections | 38187 | |
| <I/σ(I)> | 56.3 | |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 14.1 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 295 | HAMPTON RESEARCH INDEX SCREEN, SOLUTION #85: MGCL2, PEG 3350, TRIS BUFFER PH 8.5, VAPOR DIFFUSION, temperature 295K |
