2P3N
Thermotoga maritima IMPase TM1415
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-07-13 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.024, 103.873, 80.512 |
| Unit cell angles | 90.00, 101.93, 90.00 |
Refinement procedure
| Resolution | 42.000 - 2.200 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Homology built model from human AF and MJ IMPases |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.367 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.058 | 0.410 |
| Number of reflections | 46203 | |
| <I/σ(I)> | 14.1 | 2.1 |
| Completeness [%] | 97.4 | 95.5 |
| Redundancy | 3.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 15% PEG 400, 0.2M magnesium sulfate, 50mM ammonium sulfate, 50mM cacodylate, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
