2P24
I-Au/MBP125-135
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 103.656, 103.656, 97.246 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.370 - 2.150 |
| R-factor | 0.21138 |
| Rwork | 0.209 |
| R-free | 0.25996 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k2d |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.734 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.089 | 0.289 |
| Number of reflections | 29462 | |
| <I/σ(I)> | 18.7 | 5.99 |
| Completeness [%] | 99.9 | |
| Redundancy | 13.8 | 13.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291.15 | 0.1 M NaOAc, 25% (w/v) PEG 1000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K |
