2OZ7
Crystal structure of the human androgen receptor T877A mutant ligand-binding domain with cyproterone acetate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 2005-07-21 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.504, 65.820, 71.506 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.960 - 1.800 |
R-factor | 0.253 |
Rwork | 0.253 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2axa |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.960 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.075 | 0.298 |
Number of reflections | 24416 | |
<I/σ(I)> | 8.8 | 3.1 |
Completeness [%] | 96.2 | 71.8 |
Redundancy | 2.96 | 2.19 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.6 M sodium citrate, 0.1 M Hepes pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |