2OXQ

Structure of the UbcH5 :CHIP U-box complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-07
Detector	NOIR-1
Wavelength(s)	1.24
Spacegroup name	P 21 21 21
Unit cell lengths	79.042, 93.399, 144.009
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.230 - 2.900
Rwork	0.240
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f42 2esk
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.080	0.466
Number of reflections	24283
<I/σ(I)>	8.5	1.6
Completeness [%]	99.9	100
Redundancy	3.4	3.42

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	1.65M Ammonium Sulfate, 0.09M Bistris, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K