2OXE
Structure of the Human Pancreatic Lipase-related Protein 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-19 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 216.922, 216.922, 123.618 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.880 - 2.800 |
| R-factor | 0.22432 |
| Rwork | 0.222 |
| R-free | 0.26093 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bu8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.142 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 36137 | |
| <I/σ(I)> | 12.7 | 2.76 |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 298 | 1.17M Ammonium sulfate, 0.1M Sodium cacodylate pH 5.5, 0.04M NaCl, cryoprotected with 15% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 5.50 |
