2OX8
Human Scavenger Receptor C-type Lectin carbohydrate-recognition domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 |
| Unit cell lengths | 80.422, 80.422, 67.159 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.920 - 2.500 |
| Rwork | 0.233 |
| R-free | 0.30800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Phasing software | COMO |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.900 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 16533 | |
| Completeness [%] | 98.4 | 99.5 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | The protein solution contained 10mg ml-1 protein, 8mM CaCl2 , 8mM Tris pH=7.8, 20mM NaCl and 10mM Lewisx. The reservoir solution contained 8% Peg 8K, 0.2M ZnAc2 and 0.1 M Tris, pH 7.0. , VAPOR DIFFUSION, HANGING DROP, temperature 298K |
