2OV2
The crystal structure of the human RAC3 in complex with the CRIB domain of human p21-activated kinase 4 (PAK4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.96870 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.843, 73.859, 133.840 |
| Unit cell angles | 88.58, 87.76, 70.64 |
Refinement procedure
| Resolution | 62.110 - 2.100 |
| R-factor | 0.1745 |
| Rwork | 0.172 |
| R-free | 0.22453 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g0n |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.485 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.110 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.147 | 0.602 |
| Number of reflections | 106111 | |
| <I/σ(I)> | 7.8 | 1.9 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.2M Ammonium acetate, 0.1M Citrate, 30% PEG 4000, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
