2OTV
Crystal structure of the complex formed between bovine trypsin and nicotinamide at 1.56 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 292 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54132 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.831, 58.579, 67.461 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.280 - 1.560 |
| R-factor | 0.16883 |
| Rwork | 0.168 |
| R-free | 0.18586 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ayw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.313 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.280 | 1.600 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Number of reflections | 29751 | |
| Completeness [%] | 99.0 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | Ammonium sulphate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
