2OTC
ORNITHINE TRANSCARBAMOYLASE COMPLEXED WITH N-(PHOSPHONACETYL)-L-ORNITHINE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 1997-02-01 |
Detector | SIEMENS |
Spacegroup name | P 1 |
Unit cell lengths | 104.030, 114.690, 93.780 |
Unit cell angles | 86.99, 93.11, 118.81 |
Refinement procedure
Resolution | 15.000 - 2.800 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1otc |
RMSD bond length | 0.010 |
RMSD bond angle | 23.480 * |
Data reduction software | XENGEN |
Data scaling software | XENGEN |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 3.000 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.090 | 0.262 |
Number of reflections | 53778 | |
<I/σ(I)> | 7 | |
Completeness [%] | 58.0 | 20 |
Redundancy | 1.8 | 1.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | PALO | 1 (mg/ml) | |
3 | 1 | drop | HEPES | 10 (mM) | |
4 | 1 | reservoir | PEG3350 | 14 (%) | |
5 | 1 | reservoir | sodium acetate | 90 (mM) | |
6 | 1 | reservoir | 70 (mM) | ||
7 | 1 | reservoir | MPD | 2 (%) | |
8 | 1 | reservoir | Tris-HCl | 45 (mM) |