2OT3

Crystal structure of rabex-5 VPS9 domain in complex with nucleotide free RAB21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 21 21 2
Unit cell lengths	71.897, 113.400, 60.533
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.19099
Rwork	0.188
R-free	0.24272
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z0i 1txu
RMSD bond length	0.012
RMSD bond angle	1.230
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.154
High resolution limit [Å]	2.100	2.100
Number of reflections	40412
<I/σ(I)>	22	4
Completeness [%]	99.2
Redundancy	6	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	10% PEG 6000, 0.2M MgCl2, 0.05 NaMES Microseeded, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K