2OQN
High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-01-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.7463 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.968, 57.968, 150.716 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.990 - 1.900 |
| R-factor | 0.17764 |
| Rwork | 0.176 |
| R-free | 0.21623 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lxz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.322 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 20909 | |
| <I/σ(I)> | 32 | 5 |
| Completeness [%] | 99.4 | 93.9 |
| Redundancy | 12.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Crystallized in a polycarbonate capillary by vapor diffusion | 7 | 293 | 0.9 M sodium potassium tartrate, pH 7.0, Crystallized in a polycarbonate capillary by vapor diffusion , temperature 293K |
