2ONM
Human Mitochondrial Aldehyde Dehydrogenase Asian Variant, ALDH2*2, complexed with NAD+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-03-29 |
Detector | NOIR-1 |
Wavelength(s) | 1.072 |
Spacegroup name | P 1 |
Unit cell lengths | 96.201, 104.854, 162.359 |
Unit cell angles | 78.99, 82.14, 88.55 |
Refinement procedure
Resolution | 44.010 - 2.500 |
R-factor | 0.233 |
Rwork | 0.230 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1o05 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.400 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.950 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.058 | 0.325 |
Total number of observations | 40530 | |
Number of reflections | 208258 | |
<I/σ(I)> | 9.1 | 2 |
Completeness [%] | 97.6 | 97 |
Redundancy | 1.95 | 1.95 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.8 | 293 | 100 mM ACES (N-[2-Acetamido]-2-aminoethane sulfonic acid), 10mM MgCl2, 100 mM Guanidine HCl, 15% w/v PEG 6000, 8mM DTT, pH 6.8, VAPOR DIFFUSION, temperature 293K |