2OLE
Crystal Structure Of Human Dipeptidyl Peptidase IV (DPPIV) Complex With Cyclic Hydrazine Derivatives
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 295 |
Detector technology | CCD |
Collection date | 2005-04-02 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.95078 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.858, 126.545, 112.137 |
Unit cell angles | 90.00, 99.64, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.400 |
R-factor | 0.22 |
Rwork | 0.216 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.830 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 113734 | |
<I/σ(I)> | 4.9 | |
Completeness [%] | 81.6 | 93.8 |
Redundancy | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 24% PEG 4000, 0.1M Tris, 0.3M Sodium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |