2OKR
Crystal Structure of the P38a-MAPKAP kinase 2 Heterodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2005-04-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 |
| Unit cell lengths | 81.550, 81.550, 121.360 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.480 - 2.000 |
| R-factor | 0.2385 |
| Rwork | 0.239 |
| R-free | 0.27210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lez |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.193 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.2SSI) |
| Phasing software | MOLREP |
| Refinement software | autoBUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.780 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.059 | 0.364 |
| Total number of observations | 13657 | |
| Number of reflections | 60104 | |
| <I/σ(I)> | 9 | 2.2 |
| Completeness [%] | 98.6 | 90.7 |
| Redundancy | 3.06 | 2.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 25% Peg 3350, 250 mM Sodium Formate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
