2OKO

Z. mobilis tRNA guanine transglycosylase E235Q mutant apo-structure at pH 5.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Detector technology	IMAGE PLATE
Collection date	2006-12-13
Detector	RIGAKU RAXIS IV
Spacegroup name	C 1 2 1
Unit cell lengths	90.407, 65.194, 70.514
Unit cell angles	90.00, 96.36, 90.00

Refinement procedure

Resolution	10.000 - 1.500
R-factor	0.1256
Rwork	0.125
R-free	0.17390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y5x
RMSD bond length	0.011
RMSD bond angle	0.030
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	SHELXL-97

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.500
Rmerge	0.122
Number of reflections	210241
<I/σ(I)>	33
Completeness [%]	91.5
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5		pH 5.5