2OKC
Crystal structure of Type I restriction enzyme StySJI M protein (NP_813429.1) from Bacteroides thetaiotaomicron VPI-5482 at 2.20 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-17 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837, 0.97899, 0.97935 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.440, 85.850, 152.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.620 - 2.200 |
| R-factor | 0.185 |
| Rwork | 0.182 |
| R-free | 0.23200 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.565 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.620 | 28.600 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.720 | 2.200 |
| Rmerge | 0.049 | 0.021 | 0.378 |
| Number of reflections | 46716 | ||
| <I/σ(I)> | 11.63 | 31.3 | 2.2 |
| Completeness [%] | 88.9 | 85.3 | 86.2 |
| Redundancy | 3.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.33 | 277 | NANODROP, 7.5% 2-propanol, 20.0% polyethylene glycol 4000, 0.1M HEPES pH 7.33, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
