2OJT
Structure and mechanism of kainate receptor modulation by anions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.92017 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 97.598, 97.732, 128.688 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.830 - 1.950 |
| R-factor | 0.19223 |
| Rwork | 0.190 |
| R-free | 0.23291 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f34 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.331 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.048 | 0.380 |
| Number of reflections | 44649 | |
| <I/σ(I)> | 10.8 | 3.8 |
| Completeness [%] | 97.9 | 99 |
| Redundancy | 7.7 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 293 | 19% PEG 1000, 120 mM Br, 100 mM Tris-HCl, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
