2OJ9
Structure of IGF-1R kinase domain complexed with a benzimidazole inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-19 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.271, 44.289, 65.744 |
| Unit cell angles | 90.00, 99.05, 90.00 |
Refinement procedure
| Resolution | 13.210 - 2.000 |
| R-factor | 0.217 |
| Rwork | 0.214 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.017 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | BUSTER-TNT (1.9.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.091 | 0.240 |
| Number of reflections | 21556 | |
| <I/σ(I)> | 20.9 | 2.6 |
| Completeness [%] | 96.5 | 78.2 |
| Redundancy | 5.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 277 | VAPOR DIFFUSION, HANGING DROP, temperature 277K |
