2OGO
The crystal structure of the large ribosomal subunit from Deinococcus radiodurans complexed with the pleuromutilin derivative retapamulin (SB-275833)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Collection date | 2005-12-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979290 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 170.115, 405.873, 695.243 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.960 - 3.660 |
| Rwork | 0.260 |
| R-free | 0.33400 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.790 |
| High resolution limit [Å] | 3.660 | 3.660 |
| Rmerge | 0.190 | 0.791 |
| Number of reflections | 243598 | |
| <I/σ(I)> | 9.1 | 1.4 |
| Completeness [%] | 93.1 | 70 |
| Redundancy | 8.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | Ethanol, Dimethylhexanediol, MgCl2, HEPES, NH4Cl, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Ethanol | ||
| 10 | 1 | 2 | HEPES | ||
| 11 | 1 | 2 | NH4Cl | ||
| 2 | 1 | 1 | Dimethylhexanediol | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | HEPES | ||
| 5 | 1 | 1 | NH4Cl | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | Ethanol | ||
| 8 | 1 | 2 | Dimethylhexanediol | ||
| 9 | 1 | 2 | MgCl2 |
