2OGA
X-ray crystal structure of S. venezuelae DesV in complex with ketimine intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-06 |
| Detector | BRUKER PROTEUM |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.730, 143.390, 92.560 |
| Unit cell angles | 90.00, 108.49, 90.00 |
Refinement procedure
| Resolution | 36.000 - 2.050 |
| R-factor | 0.169 |
| Rwork | 0.165 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 2.150 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 80130 | |
| <I/σ(I)> | 14.3 | 3 |
| Completeness [%] | 84.6 | 71.4 |
| Redundancy | 4.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 6 | 298 | 12% PEG 3400, 1% ethylene glycol, 50 mM MES, 75 mM NaCl, pH 6.0, batch, temperature 298K |
