2OE8
1.8 A X-ray crystal structure of Apramycin complex with RNA fragment GGGCGUCGCUAGUACC/CGGUACUAAAAGUCGCC containing the human ribosomal decoding A site: RNA construct with 5'-overhang
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-04-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 27.858, 37.259, 85.586 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.20984 |
Rwork | 0.208 |
R-free | 0.23611 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2oe5 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.817 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.800 |
Number of reflections | 8704 |
Completeness [%] | 99.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.05M MES BUFFER, 15-20% MPD, 0.02-0.04M MAGNESIUM ACETATE, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 5.6 |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MES BUFFER | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | MAGNESIUM ACETATE | ||
4 | 1 | 2 | MPD | ||
5 | 1 | 2 | MAGNESIUM ACETATE |