2OE1

Crystal Structure of Mitochondrial Thioredoxin 3 from Saccharomyces cerevisiae (reduced form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-12-31
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54179
Spacegroup name	P 31
Unit cell lengths	49.575, 49.575, 94.554
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.410 - 2.100
R-factor	0.16258
Rwork	0.160
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1eru
RMSD bond length	0.017
RMSD bond angle	1.565
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.410	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.111	0.393
Number of reflections	14192
<I/σ(I)>	14.4	3.6
Completeness [%]	93.5	91.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	0.2M ammonium sulfate, 0.1M sodium acetate, 8% PEG 4000, 15% glycerol, 8mM DTT, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K