2OBV
Crystal structure of the human S-adenosylmethionine synthetase 1 in complex with the product
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-01 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54180 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 66.404, 95.016, 115.773 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.050 |
| R-factor | 0.1411 |
| Rwork | 0.139 |
| R-free | 0.18315 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1QM4 2HJ2 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.383 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.090 | 0.284 |
| Number of reflections | 21754 | |
| <I/σ(I)> | 9.7 | 5.1 |
| Completeness [%] | 93.5 | 93.5 |
| Redundancy | 5.2 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 200 mM NaF, 20 % PEG 3350, 10 % ethylene glycol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
