2O93
Crystal structure of NFAT bound to the HIV-1 LTR tandem kappaB enhancer element
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-14 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.691, 95.299, 159.227 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.050 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.28300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p7h |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.160 |
High resolution limit [Å] | 3.050 | 6.550 | 3.050 |
Rmerge | 0.129 | 0.050 | 0.538 |
Number of reflections | 26944 | ||
<I/σ(I)> | 11 | ||
Completeness [%] | 97.9 | 99.8 | 83.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 50mM Hepes pH 7.0, 15mM Magnesium Acetate, 250mM Ammonium Acetate, 7.5% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
2 | 1 | 1 | Ammonium Acetate | ||
3 | 1 | 1 | Magnesium Acetate | ||
4 | 1 | 1 | Hepes | ||
5 | 1 | 2 | PEG 4000 | ||
6 | 1 | 2 | Ammonium Acetate | ||
7 | 1 | 2 | Magnesium Acetate |