2O7M
The C-terminal loop of the homing endonuclease I-CreI is essential for DNA binding and cleavage. Identification of a novel site for specificity engineering in the I-CreI scaffold
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 43 |
| Unit cell lengths | 69.088, 69.088, 93.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.540 - 2.000 |
| R-factor | 0.18634 |
| Rwork | 0.184 |
| R-free | 0.23464 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g9z |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.137 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 25943 |
| Completeness [%] | 93.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
