2O72
Crystal Structure Analysis of human E-cadherin (1-213)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-03 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.341, 41.077, 59.542 |
| Unit cell angles | 90.00, 111.15, 90.00 |
Refinement procedure
| Resolution | 39.100 - 2.000 |
| R-factor | 0.193 |
| Rwork | 0.191 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1edh |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.445 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.100 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.073 | 0.323 |
| Number of reflections | 20837 | |
| <I/σ(I)> | 9.3 | 2.5 |
| Completeness [%] | 98.0 | 77.8 |
| Redundancy | 6.8 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 10% PEG 8000, 0.2M calcium chloride, 5% DMSO, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 4K, temperature 277K |
