2O6V
Crystal structure and solution NMR studies of Lys48-linked tetraubiquitin at neutral pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Collection date | 2004-08-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9786 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 59.100, 77.080, 139.360 |
| Unit cell angles | 90.00, 90.32, 90.00 |
Refinement procedure
| Resolution | 38.000 - 2.200 |
| R-factor | 0.24 |
| Rwork | 0.222 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aar |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.460 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 29408 | |
| <I/σ(I)> | 22.5 | 2.8 |
| Completeness [%] | 93.4 | 93.7 |
| Redundancy | 3.2 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 2M ammonium sulfate, 4% PEG 400, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
