2O6Q
Structural diversity of the hagfish Variable Lymphocyte Receptors A29
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2006-06-09 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97175 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 95.226, 95.226, 77.556 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.930 - 2.500 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ozn |
RMSD bond length | 0.020 |
RMSD bond angle | 2.000 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 13115 | |
<I/σ(I)> | 17.4 | 2.3 |
Completeness [%] | 91.0 | 61.5 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 33% PEG 1000, 0.1M Bis-Tris, 0.2M magnesium chloride, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |