2O5J
Crystal structure of the T. thermophilus RNAP polymerase elongation complex with the NTP substrate analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-25 |
Detector | MARMOSAIC 300 mm CCD |
Spacegroup name | P 41 |
Unit cell lengths | 152.340, 152.340, 524.567 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 3.000 |
R-factor | 0.2293 |
Rwork | 0.225 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o5i |
RMSD bond length | 0.015 |
RMSD bond angle | 1.820 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.077 | 0.441 |
Number of reflections | 211633 | |
<I/σ(I)> | 11.7 | 2.1 |
Completeness [%] | 89.3 | 75.5 |
Redundancy | 3.4 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 30% MPD, 0.1M Na cacodylate, 0.2M Mg acetate, 2mM AMPcPP, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | Na cacodylate | ||
3 | 1 | 1 | Mg acetate | ||
4 | 1 | 1 | AMPcPP | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | MPD | ||
7 | 1 | 2 | Na cacodylate | ||
8 | 1 | 2 | Mg acetate | ||
9 | 1 | 2 | H2O |