2O52

Crystal structure of human RAB4B in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-10-25
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	67.528, 67.528, 179.955
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
Rwork	0.213
R-free	0.26930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bme
RMSD bond length	0.016
RMSD bond angle	1.409
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.280
High resolution limit [Å]	2.200	4.730	2.200
Rmerge	0.119	0.048	0.976
Number of reflections	40474	4073	4044
<I/σ(I)>	9
Completeness [%]	100.0
Redundancy	6.2	6.6	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	18% PEG8000, 0.2M magnesium acetate, 0.1M sodium cacodylate, pH 6.5, vapor diffusion, sitting drop, temperature 291K