2O4G
Structure of TREX1 in complex with a nucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.183, 81.459, 93.127 |
| Unit cell angles | 90.00, 103.22, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.350 |
| R-factor | 0.20587 |
| Rwork | 0.203 |
| R-free | 0.25456 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y97 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.360 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 40389 | |
| <I/σ(I)> | 10 | 3.7 |
| Completeness [%] | 98.2 | 87.6 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 22% PEG 3350, 0.1M MES, 0.2M lithium sulfate , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
