2O3F
Structural Genomics, the crystal structure of the N-terminal domain of the putative transcriptional regulator ybbH from Bacillus subtilis subsp. subtilis str. 168.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-07 |
Detector | SBC-3 |
Wavelength(s) | 0.97904 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 58.898, 48.060, 58.854 |
Unit cell angles | 90.00, 119.92, 90.00 |
Refinement procedure
Resolution | 25.520 - 1.750 |
R-factor | 0.18282 |
Rwork | 0.181 |
R-free | 0.21452 |
Structure solution method | SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.725 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.520 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.069 | 0.478 |
Number of reflections | 28223 | |
<I/σ(I)> | 40.35 | 2.42 |
Completeness [%] | 98.2 | 86.7 |
Redundancy | 6.7 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1M Sodium acetate, 2M Ammonium sulfate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |