2O39

Human Adenovirus type 11 knob in complex with domains SCR1 and SCR2 of CD46 (membrane cofactor protein, MCP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-10
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 3
Unit cell lengths	106.140, 106.140, 68.320
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 2.850
R-factor	0.238
Rwork	0.238
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Human Adenovirus type 11 (unpublished) CD46 SCR1 and SCR2
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.950
High resolution limit [Å]	2.850	2.850
Rmerge	0.138	0.325
Number of reflections	18738
<I/σ(I)>	7.23	2.64
Completeness [%]	93.1	83
Redundancy	4.1	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	35% PEG 200, 200mM CaCl2, 100mM Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K