2NZT

Crystal structure of human hexokinase II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-08-15
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	94.890, 129.338, 187.232
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.450
Rwork	0.230
R-free	0.28700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cza
RMSD bond length	0.017
RMSD bond angle	1.427
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.540
High resolution limit [Å]	2.450	5.270	2.450
Rmerge	0.082	0.043	0.671
Number of reflections	85698	8929	8448
<I/σ(I)>	15.3
Completeness [%]	99.8
Redundancy	5.5	6.6	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	16% PEG3350, 0.2M sodium malonate, 0.1M BTP, 10% ethylene glycol, 0.001M DDT, pH 8.5, vapor diffusion, sitting drop, temperature 291K