2NZD
Nucleosome core particle containing 145 bp of DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 98 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-05 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.617, 109.856, 181.217 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.650 |
| R-factor | 0.234 |
| Rwork | 0.233 |
| R-free | 0.28300 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1kx3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.450 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.700 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.065 | 0.480 |
| Total number of observations | 27467 | |
| Number of reflections | 56193 | |
| <I/σ(I)> | 20.9 | 2.7 |
| Completeness [%] | 91.0 | 63.9 |
| Redundancy | 6.3 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 85 mM MnCl2, 60 mM KCl, 20 mM K-Cacodylate, 4 mg/ml NCP over well with 1/2 conc., pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MnCl2 | ||
| 2 | 1 | 1 | KCl | ||
| 3 | 1 | 1 | K-Cacodylate | ||
| 4 | 1 | 1 | HOH | ||
| 5 | 1 | 2 | MnCl2 | ||
| 6 | 1 | 2 | KCl | ||
| 7 | 1 | 2 | K-Cacodylate | ||
| 8 | 1 | 2 | HOH |
