2NY7

HIV-1 gp120 Envelope Glycoprotein Complexed with the Broadly Neutralizing CD4-Binding-Site Antibody b12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-13
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 65 2 2
Unit cell lengths	101.955, 101.955, 298.294
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.450 - 2.300
R-factor	0.19601
Rwork	0.193
R-free	0.25503
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb entries 1HZH 1gc1
RMSD bond length	0.004
RMSD bond angle	0.852
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.096	0.057	0.755
Number of reflections	40798
<I/σ(I)>	9.8
Completeness [%]	97.2	98.7	77.5
Redundancy	8.8	9.7	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1