2NX3
Structural and mechanistic changes along an engineered path from metallo to non-metallo KDO8P synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 76.381, 198.528, 125.834 |
Unit cell angles | 90.00, 94.19, 90.00 |
Refinement procedure
Resolution | 29.790 - 2.100 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fwn |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.800 |
High resolution limit [Å] | 2.100 |
Rmerge | 0.069 |
Number of reflections | 206008 |
<I/σ(I)> | 6.9 |
Completeness [%] | 0.9 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |