2NUE
Crystal structure of RNase III from Aquifex aeolicus complexed with ds-RNA at 2.9-Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.596, 101.657, 67.082 |
| Unit cell angles | 90.00, 103.69, 90.00 |
Refinement procedure
| Resolution | 29.110 - 2.900 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rc7 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.072 | 0.160 |
| Number of reflections | 13199 | |
| <I/σ(I)> | 13.3 | 3.4 |
| Completeness [%] | 89.1 | 53.4 |
| Redundancy | 2.6 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M NaCl + 16% PEG3350 in 0.1 M Tris-Cl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NaCl | ||
| 2 | 1 | 1 | PEG3350 | ||
| 3 | 1 | 1 | Tris-Cl | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | NaCl | ||
| 6 | 1 | 2 | PEG3350 | ||
| 7 | 1 | 2 | H2O |
