2NMV

Damage detection by the UvrABC pathway: Crystal structure of UvrB bound to fluorescein-adducted DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.980
Spacegroup name	P 21 21 21
Unit cell lengths	74.410, 95.597, 97.878
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.360 - 2.950
R-factor	0.22706
Rwork	0.224
R-free	0.28074
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2d7d
RMSD bond length	0.016
RMSD bond angle	1.713
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.360	3.030
High resolution limit [Å]	2.950	2.950
Rmerge	0.094	0.369
Number of reflections	15109
<I/σ(I)>	15.9	5.2
Completeness [%]	99.1	99.3
Redundancy	5.3	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5	289	18-20% PEG 20000, 0.1M pH 8.5 Tris-Hcl, microbatch, temperature 289K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 20000
2	1	1	Tris-Hcl
3	1	2	PEG 20000