2NL8
The origin binding domain of the SV40 large T antigen bound non specifically to a 17 bp palindrome DNA (sites 1 and 3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-05-26 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 43.870, 43.870, 238.308 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.300 |
| R-factor | 0.23999 |
| Rwork | 0.237 |
| R-free | 0.29895 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1IPR |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.191 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 11307 | |
| <I/σ(I)> | 18.1 | 2.1 |
| Completeness [%] | 94.0 | 93 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297 | 20 mM NaAcetate, 20% PEG 3000 + 4% PEG 600, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NaAcetate | ||
| 2 | 1 | 1 | PEG 3000 | ||
| 3 | 1 | 1 | PEG 600 | ||
| 4 | 1 | 2 | NaAcetate | ||
| 5 | 1 | 2 | PEG 3000 | ||
| 6 | 1 | 2 | PEG 600 |
