2JLW

Dengue virus 4 NS3 helicase in complex with ssRNA2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Spacegroup name	C 1 2 1
Unit cell lengths	132.432, 104.628, 72.317
Unit cell angles	90.00, 117.67, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.20236
Rwork	0.199
R-free	0.27007
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jlq
RMSD bond length	0.008
RMSD bond angle	1.212
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.400	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.150	0.580
Number of reflections	26932
<I/σ(I)>	11	1.9
Completeness [%]	100.0	100
Redundancy	4.4	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1