2JLV

Dengue virus 4 NS3 helicase in complex with ssRNA and AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Spacegroup name	C 1 2 1
Unit cell lengths	132.660, 105.192, 72.435
Unit cell angles	90.00, 117.66, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.19349
Rwork	0.190
R-free	0.24801
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jlu
RMSD bond length	0.010
RMSD bond angle	1.338
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	2.400	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.130	0.500
Number of reflections	44454
<I/σ(I)>	11.6	2.7
Completeness [%]	99.3	98.6
Redundancy	4.9	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1